84-2155-89　Heregulin beta-1 (Neuregulin-1, Acetylcholine Receptor-inducing Activity, Breast Cancer Cell Differentiation Factor P45, Glial Growth Factor, Heregulin, Neu Differentiation Factor, Sensory and Motor Neuron-derived Factor) (HRP) 100ul　143581-HRP

特徴

• Neuregulin 1 (NRG1) was originally identified as a 44kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues.
• It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing.
• These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF).
• They are tissue-specifically expressed and differ significantly in their structure.
• The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains.
• GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms.
• The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors.
• Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentiation of epithelial, neuronal, glial, and other types of cells.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Neutralization.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (FFPE): Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.