84-2155-82　Interleukin 6 (IL-6) (HRP) 100ul　I8428-25A-HRP

特徴

• Interleukin-6 (IL-6) is a pleotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses.
• IL-6 is produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes.
• It has diverse biological functions.
• It stimulated B cell differentiation and antibody production.
• It synergizes with IL-3 in megakaryocyte development and platelet production.
• It induces expression of hepatic acute phase proteins.
• It regulated bone metabolism.
• IL-6 signals through the IL-6 receptor system that consists of two chains: IL-6Ra and gp130.
• Applications:Suitable for use in Neutralization, ELISA and Western Blot.
• Other applications have not been tested.
• Recommended Dilution:Neutralization: 0.2-0.4ug/ml yields one-half maximal inhibition [ND50] of the biological activity (1ng/ml)
• Direct ELISA: 0.5ug/ml (100ul/well).
• The detection limit is 0.2-0.4ng/well
• Western Blot: 0.1-0.2ug/ml.
• The detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
• Optimal dilutions to be determined by the researcher
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.