84-2155-57　Bone Morphogenetic Protein 2 (BMP2) (HRP) 100ul　B2553-07C-HRP

特徴

• Human Bone Morphogenetic Protein 2 (BMP2) is a disulfide-bonded homodimeric protein with an apparent molecular weight of 26kD.
• BMP2 regulates similiarly to its nearest homologue BMP-4 diverse fundamental processes during embryonic development: BMP2 and other BMP proteins have great potential for medical therapeutic applications, in particular because they allow or at least accelerate the ossification of extensive bone lesions.
• BMP2 lacks the natural N-terminus which results in a 15-20 fold increase of specific activity.
• BMP2 is a heparin binding protein.
• Applications:Suitable for use in ELISA, Western Blot and Neutralization.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.5-2ug/ml (100ul/well); Detects 0.2-0.4ng/well of recombinant hBMP-2
• Western Blot: 0.1-0.2ug/ml; Detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions
• Neutralization: 1.3-1.9ug/ml; Yields one-half maximal inhibition [ND50] of the biological activity of hBMP-2 (200ng/ml)
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.