Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors.
It is important for proliferation during certain stages of B-cell maturation.
Applications:Suitable for use in ELISA, Western Blot and Neutralization.
Other applications not tested.
Recommended Dilution:ELISA (Sandwich): To detect Rat IL-7 by sandwich ELISA (Sandwich) (using 100ul/well antibody solution) a concentration of 0.5-2ug/ml of this antibody is required.
This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-7 as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant Rat IL-7
Western Blot: To detect Rat IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml.; When used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-7 is 1.5-3ng/lane, under either reducing or non-reducing conditions
Neutralization: To yield one-half maximal inhibition of the biological activity of Rat IL-7 (5ng/ml), a concentration of 0.14-0.21ug/ml of this antibody is required
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
