84-2154-44　NP-1 (alpha Defensin-1) (APC) 100ul　N5377-01-APC

特徴

• Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans.
• Defensins, cationic/polar peptides (30-35 aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely b-sheet structure.
• In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms.
• Defensins are classified into two families designated alpha and beta based on distinctive, although similar, tri-disulfide linkages in the peptides.
• b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides.
• In humans, six a-defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two b-defensins, HBD-1 and HBD-2, have been identified.
• Alpha-defensins are encoded by genes designated DEFA1-6, whereas human b-defensins are encoded by the DEFB1 and DEFb2 genes.
• HD1-4 are expressed in neutrophils, whereas HD5 and HD6 are expressed in epithelial cells of the intestinal and reproductive tract.
• HD1-3/HNP1-3 make up about 30% of the neutrophil's total granule protein.
• The HNPs are 29 to 30 amino acids long and are identical in sequence except at the N-terminal amino acid.
• Applications:Suitable for use in ELISA, Neutralization and Western Blot.
• Other applications have not been tested.
• Recommended Dilution:  Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: APC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.