CD80, also known as BB1/B7-1, is a 60kD type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors.
It is expressed on activated B cells, T cells, monocytes and dendritic cells.
It is a ligand of the two T cell costimulatory molecules, CD28 and CD152 (CTLA-4).
The interaction of CD80 (or CD86) with CD28 provides a costimulatory signal for T cells activated through the CD3/TCR complex.
Applications: ELISA: To detect mB-71 by direct ELISA (using 100ul/well antibody solution) a concentration of at least 0.5ug/ml of this antibody is required.
This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant mB-71.
Western Blot: To detect mB-71 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant mB-71 is 1.5-3ng/lane, under either reducing or non-reducing conditions.
Storage and Stability:May be stored at 4°C before opening.
DO NOT FREEZE! Stable at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Further dilutions can be made in assay buffer.
Allophycocyanin (APC) is light sensitive.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Recognizes mouse CD80.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to mouse CD80 expressed in E. coli.
