84-2154-08　［取扱停止］VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF) (AP) 100ul　V2110-09A-AP

特徴

• Applications:Suitable for use in Neutralization, ELISA, Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (Formalin-fixed paraffin embedded): 5-15ug/ml with a 30 minute incubation at RT or an overnight incubation at 4ºC
• Neutralization: 0.05-0.1ug/ml yields one-half maximal inhibition [ND50] of the biological activity (10ng/ml)
• ELISA (Indirect amd Sandwich): 0.5ug/ml (100ul/well).
• Used in conjunction with compatible secondary reagents, allows detection of 0.2-0.4ng/well of recombinant hVEGF
• Western Blot: 0.1-0.2ug/ml.
• Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C before opening.
• DO NOT FREEZE! Stable at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.
• Stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.
• Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
• Note: Applications are based on unconjugated antibody.