Nerve growth factor-beta (NGF-b) is a potent neurotrophic factor which supports the growth and survivability of nerve and/or glial cells.
The active form of human beta-NGF is is a noncovalently disulfide-linked homodimer, formed by two 13.5kD polypeptide monomers (238 total aa).
The three disulfide bonds are required for biological activity.
Applications:Suitable for use in ELISA, Western Blot and Neutralization.
Other applications not tested
Recommended Dilution:ELISA: 0.5-2ug/ml.
This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human beta-NGF as a detection antibody, allows the detection of 0.2-0.4ng/well of recombinant Human beta-NGF
Western Blot: 0.1-0.2ug/ml.
When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human beta-NGF is 1.5-3ng/lane, under either reducing or non-reducing conditions
Neutralization: To yield one-half maximal inhibition of the biological activity of Human beta-NGF (1ng/ml), a concentration of 0.05-0.08ug/ml of this antibody is required
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C before opening.
DO NOT FREEZE! Stable at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP).
