Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors.
It is important for proliferation during certain stages of B-cell maturation.
Applications:Suitable for use in ELISA, Western Blot and Neutralization.
Other applications not tested.
Recommended Dilution:ELISA (Sandwich): To detect Rat IL-7 by sandwich ELISA (Sandwich) (using 100ul/well antibody solution) a concentration of 0.5-2ug/ml of this antibody is required.
This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-7 as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant Rat IL-7
Western Blot: To detect Rat IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml.; When used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-7 is 1.5-3ng/lane, under either reducing or non-reducing conditions
Neutralization: To yield one-half maximal inhibition of the biological activity of Rat IL-7 (5ng/ml), a concentration of 0.14-0.21ug/ml of this antibody is required
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C before opening.
DO NOT FREEZE! Stable at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP).
