Histones are a group of similar, small, highly conserved nuclear proteins that bind to DNA by their many basic residues.
The histones are divided into fractions, with each fraction having a distinct amino acid composition and sequence.
Histones are also classified by relatively high levels of lysine and arginine.
The presence of autoantibodies to histones are frequently found in several rheumatic disorders.
In one study, the predominant responses to histones in SLE sera were to H1, H2b, and H3.
Marked elevations of binding occurred to H1 and H2b in 33% of patients, while 25% showed higher binding to H3.
The same study showed the highest anti-histone reactivity to be in patients with rheumatoid arthritis with vasculitis, while the highest reactivity in SLE sera was in those patients with a history of photosensitivity.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:100Optimal dilutions to be determined by the researcher.
Storage amd Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ul
Host：human
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder. Reconstitute with 500ul ddH20.
