Specificity is verified by identity of precipitation with CDC-AF reference serum.
Titer will vary with test system; however, undiluted antibody shows a strong SSB precipitate band.
Applications:Suitable for use in ELISA and Immunodiffusion.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
SSB Autoantibody Activity (by ELISA):732EU/ml (lot-specific; value may differ from lot to lot).
Other Autoantibody Activity in the Serum (by ELISA):SSA: 126EU/ml SCL: 2EU/mlSm: 3EU/ml Sm/RNP: 4EU/ml Jo-1: 3EU/ml
Interpretation of ELISA Results:Negative: <16EU/ml, Equivocal: 16-20EU/ml, Positive: >20EU/ml
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:250ul
Host:human
Source Antibody:human
Grade:Purified
Purity:Purified by fractionation of delipidated and defibrinated donor human plasma
Form:Supplied as a lyophilized powder from PBS (0.01M phosphate/0.13M NaCl), pH 7.2, 0.02% sodium azide. Reconstitute with 250ul ddH2O.
Specificity:Recognizes human SSB. Undiluted antibody shows a strong SSB precipitate band. Specificity is verified by a precipitin line of identity with the CDC-AF reference serum.