Specificity is verified by identity of precipitation with CDC-AF reference serum.
Titer will vary with test system; however, undiluted antibody shows a strong Jo-1 precipitate band.
Applications:Suitable for use in ELISA and double immunodiffusion.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Titer:Immunodiffusion: 1:8
In an ELISA system designed to detect all IgG subclasses, the following purified antigens (dilution of 1:100) responded as:AntigenResult (EU/ml)SSA1 SSB1SCL4Sm1Sm/RNP2Jo-194
Interpretation of Results:<16 EU/ml: Negative16-20 EU/ml: Equivocal>20 EU/ml: Positive
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile xddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：250ul
Host：human
Source Antibody：human
Grade：Purified
Purity：Purified by delipidation, defibrination and ammonium sulfate precipitation
Form：Supplied as a lyophilized powder from 0.01M phosphate, 0.13M sodium chloride, 0.02% sodium azide. Reconstitute with 250ul sterile ddH2O.
Specificity：Recognizes Jo-1. Shows only the Jo-1 precipitate band when tested using double immunodiffusion against rabbit thymus extract. Undiluted antibody shows a strong Jo-1 precipitate band.
