This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation.
This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn.
This enzyme is also essential for thymocyte development.
Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells.
Two transcript variants that encode different isoforms have been found for this gene.
Applications:Suitable for use in Peptide ELISA, Immunoprecipitation and Western Blot.
Other applications not tested.
Recommended Dilution:Peptide ELISA: 1:32,000Western Blot: 0.3-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human ZAP70. This antibody is expected to recognize isoform 1 (NP_001070.2) only.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-SDGYTPEPARITSPD, from the internal region of the protein sequence according to NP_001070.2. Species sequence homology: mouse, rat, canine and bovine
