Vascular Adhesion Protein 1 (VAP-1), also called AOC3 (amine oxidase copper-containing 3) or SSAO (semicarbazide-sensitive amine oxidase) is a type II transmembrane sialoglycoprotein that is upregulated on endothelial cells by inflammation.
The 170kD disulfide-linked homodimer of 763 aa units is an ectoenzyme that deaminates amines, forming aldehydes and hydrogen peroxide.
It also mediates lymphocyte rolling, adhesion and possibly extravasation during inflammation.
Adipocytes can release a soluble form via metalloproteinase cleavage.
Human and mouse VAP-1 share 83% aa identity.
Applications:Suitable for use in Peptide ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Peptide ELISA: 1:128,000Western Blot: 0.1-0.3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human VAP1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to ESQAPKTIRDAFC, from the internal region of the protein sequence according to NP_003725.1. Species sequence homology: rat and mouse
