Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif.
The 88,000 Da precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor.
Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6,000 Da peptides.
These smaller cleavage products are named granulin A, granulin B, granulin C, etc.
Both the peptides and intact granulin protein regulate cell growth.
However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth.
Granulin family members are important in normal development, wound healing, and tumorigenesis.
GRN is expressed in myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines.
present in inflammatory cells and bone marrow.
highest levels in kidney.
Applications:Suitable for use in Peptide ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Peptide ELISA: 1:8000Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human Progranulin. This antibody is expected to recognize the C-Terminal part of Granulin-3.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa248-259 from sequence PQDTVCDLIQSK, at the internal of the protein sequence according to NP_002078.1.
