Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs).
Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes.
Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression.
Recruitment of SETDB1 induces heterochromatinization.
May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1.
Also corepressor for ERBB4.
Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation.
May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1.
Important regulator of CDKN1A/p21(CIP1).
Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger.
Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity.
Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6.
Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors.
Applications:Suitable for use in Peptide ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Peptide ELISA: 1:2000Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human KAP1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-KQFNKLTEDKADVQ, from the internal region of the protein sequence according to NP_005753.1. Species sequence homology: mouse, rat and canine
