Suppresses hypoxia-inducible expression of HIF1A and EPAS1.
Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters.
The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE.
Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription.
Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription.
Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia.
Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity.
Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis.
Functions as an inhibitor of angiogenesis in the cornea By similarity.
Applications:Suitable for use in Peptide ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Peptide ELISA: 1:128,000Immunohistochemistry: 4-6ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human HIF3A. This antibody is expected to recognize isoform a and c (NP_690007.1; NP_690008.2).
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to QDHRSTTELRKEKS-C, from the N-terminus of the protein sequence according to NP_690007.1; NP_690008.2. Species sequence homology: mouse and rat
