CTDSP1 is a class 2C phosphatase with activity dependent on the conserved DxD motif.
Expression of CTDSP1 inhibited activated transcription from several promoter-reporter gene constructs, but expression of a mutant lacking phosphatase activity enhanced transcription.
Neuronal gene transcription is repressed in nonneuronal cells by the repressor element-1 (RE1)-silencing transcription factor/neuron-restrictive silencer factor (REST/NRSF; 600571) complex.
REST/NRSF recruits SCPs to neuronal genes that contain RE1 elements, leading to neuronal gene silencing in nonneuronal cells.
Phosphatase-inactive forms of SCP interfere with REST/NRSF function and promote neuronal differentiation of P19 stem cells.
Likewise, small interfering RNA directed to the single Drosophila SCP unmasks neuronal gene expression in S2 cells.
Thus, SCP activity is an evolutionarily conserved transcriptional regulator that acts globally to silence neuronal genes.
Applications:Suitable for use in Peptide ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Peptide ELISA: 1:16,000Western Blot: 0.1-0.3ug/mlImmunohistochemistry: 5-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes CTDSP1. This antibody is expected to recognize both reported isoforms (NP_067021 and NP_872580). Species Crossreactivity: human and mouse
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to EAKAQDSDKIC, from the internal region of the protein sequence according to NP_067021.1; NP_872580.1. Species sequence homology: rat, canine and bovine
