84-2148-47　ATP5A1 (ATP synthase, H+ transporting, Mitochondrial F1 complex, alpha subunit 1, Cardiac muscle, ATP5A, ATP5AL2, ATPM, MOM2, OMR, ORM, hATP1, ATP synthase alpha chain, Mitochondrial, ATP synthase subunit alpha, mitochondrial, ATP synthase, H+ transportin) 100ug　208015

特徴

• Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.
• F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk.
• During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation.
• Subunits alpha and beta form the catalytic core in F1.
• Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
• Subunit alpha does not bear the catalytic high-affinity ATP-binding sites By similarity.
• Applications:Suitable for use in Peptide ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Peptide ELISA: 1:128,000Western Blot: 0.01-0.03ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.