84-2148-23　Alpha-synuclein (NACP140) (NACP, Non A-beta component of AD amyloid, PARK1, PARK4, PD1, SNCA, Synuclein alpha-140, Synuclein, alpha (non A4 component of amyloid precursor)) 100ug　207991

特徴

• The implication of alpha synuclein in neurodegenerative diseases started after the identification of non A beta component of amyloid (NAC peptide) in Alzheimer's disease (AD) brains.
• There is now a general group of synucleinopathies which implicates alpha synuclein in Lewy body syndromes, Parkinson' disease and Alzheimer's disease.
• Certain missense mutations in the alpha synuclein gene (A53T, A30P) have been linked to the familial Parkinson's disease (PD).
• Alpha Synuclein is the major component of Lewy bodies and Lewy neuritis in sporadic PD, dementia with Lewy Bodies and Lewy Body variant of AD.
• Lewy bodies are composed of truncated and phosphorylated intermediate neurofilament proteins, alpha synuclein, ubiquitin and associated enzymes.
• The synuclein phosphoproteins (15-20kD) are small highly conserved proteins in vertebrates.
• The synuclein family includes alpha and beta synucleins and loosely related gamma synuclein and synoretin.
• The expression is abundant in neurons and typically is localized at presynaptic terminals.
• Antibodies to alpha synuclein provide a specific method to detect Lewy bodies and associated pathological mechanism of PD and AD.
• Applications:Suitable for use in Peptide ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Peptide ELISA: 1:32,000Western Blot: 0.1-0.3ug/ml.
• A band is observed at ~17kD in human brain (amygdala) lysates (calculated MW of 14.5kD)Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.