VASP (vasodilator-stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases.
VASP is an actin-associated protein involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration.
VASP promotes actin filament elongation.
It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping proteins.
VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments and it plays a role in actin-based mobility of Listeria monocytogenes in host cells.
It regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
VASP phosphorylation is used to monitor the effect of so-called antiplatelet drugs that reduce platelet reactivity and are used to prevent stent thrombosis, strokes and heart attacks in patients.
Phosphorylation of VASP at Ser157 causes a mobility shift in SDS gel electrophoresis from 46 to 50kD, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1500-1:3000Immunocytochemistry: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Positive Control (Included):Human platelet protein (500ug), supplied at 5mg/ml in SDS-stop solution (73mM Tris/HCl, pH 6.7, 100mM NaCl, 10mM DTT, 8mM EDTA, 2% SDS, 10ug/ml bromophenol blue sodium salt, 5% glycerol).
Use 5ul (25ug) per lane.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a crude serum in 0.02% sodium azide.
Specificity：Recognizes both the 46kD (Ser157 dephospho-) and 50kD (Ser157 phospho-) form of human VASP. Does not cross-react with mouse or rat VASP.
Isotype：IgG
Calc Applications Abbrev：IC IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human VASP, fused to His-Tag.
