Profilin (PFN1) is a ubiquitous small (12-15kD) phosphoinositide and poly-L-proline binding protein that plays a role in signal transduction pathways and actin filament dynamics.
There are two mammalian profilins with similar biochemical properties.
Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels also in skeletal muscle, uterus and kidney.
Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.
Profilin binding to PIP2 interferes with PIP2 hydrolysis by soluble phospholipase C-gamma, an inhibition that can be overcome by tyrosine phosphorylation of PLC-gamma.
Besides actin monomer sequestration and stimulation of actin nucleotide exchange, profilin can also promote cellular actin filament growth.
Profilin is involved in the actin dependent intracellular motility of cytopathogenic bacteria, the regulation of cell adhesion and possibly also in linking the actin cytoskeleton and endocytosis.
Profilin has been found to associate with defined complexes containing proteins such as Arp2/3 or the Rho/Rac pathways constituents ROCK-II and HEM2/NAP1.
Defects in PFN1 are the cause of amyotrophic lateral sclerosis 18 (ALS18).
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Positive Control (Included):Human platelet protein, 500ug supplied at 5mg/ml in SDS sample buffer.
Use 5ul (25ug) per lane for Western blotting of tricine gels (13% acrylamide) or 15% Laemmli gels.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 1mg/ml BSA, 0.02% sodium azide.
Specificity：Recognizes human Profilin. Species Crossreactivity: bovine, mouse, porcine and rat
