PARP-2 (ARTD2) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism.
This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000-1:5000Immunohistochemistry: 1:500-1:1000Immunocytochemistry: 1:500-1:1000Immunoprecipitation: 2ul/100ug nuclear extractOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Purity：Purified
Form：Supplied as a liquid in 0.09% sodium azide.
Specificity：Recognizes mouse PARP-2. Coss-reacts weakly with human PARP-2
Isotype：IgG
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to aa1-207 from mouse PARP-2.
