PAG [Cbp] is a ubiquitously expressed transmembrane adapter protein.
Depending on its phosphorylation status, it appears as diffuse band(s) of 80-90kD by SDS-PAGE, with phosphorylation leading to a shift towards a higher apparent molecular mass.
Tyrosine phosphorylated PAG recruits Csk to the membrane and negatively regulates Src family kinases via Csk-mediated phosphorylation.
Upon T cell receptor engagement, PAG becomes dephosphorylated, thereby displacing Csk from the membrane and enabling the activation of the Src kinases Fyn and Lck.
PAG negatively regulates TCR (T cell antigen receptor)-mediated signaling in T cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells.
It promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition.
It inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins and may be involved in cell adhesion signaling.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunocytochemistry: 1:50Immunoprecipitation: 5ug/sampleOptimal dilutions to be determined by the researcher.
Positive Control for Western Blot:Use 1.5x10e6 total splenocytes per lane.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes rat PAG. Species Crossreactivity: human and mouse
Isotype：IgG
Calc Applications Abbrev：IC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa412-424 of N-terminal rat PAG including an additional N-terminal Cys residue.
