LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall.
Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA.
However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels.
Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.
Applications:Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 3-10ug/mlELISA: 1-15ug/mlWestern Blot: 2-5ug/mlImmunohistochemistry: frozen/paraffin sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a lyophilized powder. Reconstitute with sterile dH2O to 0.1-1mg/ml.
Specificity：Recognizes mouse LYVE-1.
Isotype：IgG
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to aa24-228 from soluble mouse LYVE-1.
