84-2147-79　Lipoma Preferred Partner (LPP, LIM Domain-containing Preferred Translocation Partner in Lipoma) 50ug　207260

特徴

• Lipoma preferred partner (LPP) is encoded by the preferred fusion partner gene of the high mobility group protein HMGI-C.
• LPP is frequently affected by chromosomal translocations in a major group of soft tissue lipomas, as well as in a parosteal lipoma and in pulmonary chondroid hamartomas.
• The resulting fusion proteins comprise three DNA binding domains of HMGI-C fused to two or three C-terminal LIM domains of LPP and a reciprocal product, respectively.
• Expression of a HMGI-C/LPP fusion protein causes malignant transformation of fibroblasts.
• Similar to its relatives zyxin and TRIP (thyroid receptor interacting protein 6) / ZRP-1 (zyxin-related protein 1), LPP consists of a N-terminal proline-rich domain followed by three C-terminal LIM domains (double zinc finger structures that are involved in protein-protein interactions).
• Like the cytoskeletal protein zyxin, LPP localizes to focal adhesions and cell-cell adherens junctions.
• LPP harbors a nuclear export signal and displays transcriptional activation capacity.
• Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Immunohistochemistry: 1:500 Immunocytochemistry: 1:500Optimal dilutions to be determined by the researcher.
• Positive Control:Human skin fibroblast protein (100ug), supplied at 1mg/ml in SDS (Laemmli) sample buffer.
• Use 25ul (25ug)/lane for Western blot.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.