GPxs are glutathione peroxidases involved in balancing the H2O2 homeostasis in signaling cascades.
GPxs have been known to catalyze the reduction of H2O2 or organic hydroperoxides to H2O or the corresponding alcohols, respectively, typically using glutathione (GSH) as reductant.
GPx8 (probable glutathione peroxidase 8) is a 209-aa, 24kD protein with an N-terminal cytosolic tip, a predicted transmembrane segment and a catalytic domain located in the endoplasmic reticulum (ER) lumen.
GPx8 is a CysGPxs with low glutathione peroxidase activity.
A recent study identified GPx8 as a cellular substrate of the hepatitis C virus NS3-4A protease.
GPx8 cleavage by NS3-4A occurs at Cys11, removing the cytosolic tip of GPx8 and was observed in different experimental systems as well as in liver biopsies from patients with chronic hepatitis C.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunocytochemistry: 1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 10% glycerol.
Specificity：Recognizes human GPx8.
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant GPx8 (human) (aa 38-209) fused to a GST-tag.
