The proteolysis of collagens plays an important role in numerous physiological and pathological situations such as morphogenesis, wound healing, arthritis, arteriosclerosis and tumor metastasis.
Triple helical type I collagens are made up of two a1 (I) and one a2 (I) chains, and are found in skin, tendon, ligament and interstitial tissues.
Due to their fibrillary structure native collagens are resistant to most proteases.
They are substrates for certain matrix metalloproteinases (MMPs), which constitute a family of zinc-dependent enzymes catalyzing the degradation of extracellular matrix components.
Initial MMP-8 dependent cleavage of collagen into the characteristic ¾ and ¼ fragments has been shown to enable MMP-9 diffusion along the protein helix with preferential binding to the collagen ¾ fragment tail.
Finally, untwisting of the helix end results in the local denaturation of the triple helical structure.
Applications:Suitable for use in Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunocytochemistry: 2.5-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 1mg/ml BSA, 0.02% sodium azide.
Specificity：Recognizes Collagen Type 1. Species Crossreactivity: bovine, human, feline, canine, hamster, mouse, porcine, rat, sheep.
Isotype：IgG
Calc Applications Abbrev：IC
Calc Crossreactivity：Bo Ca Fe Hm Hu Mo Po Rt Sh
Immunogen：Synthetic peptide corresponding to the C-terminal end of the N-terminal three quarter collagen fragment (Col1 3/4).
