Maltose binding protein is a bacterial protein, which is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest.
It also allows for its easy purification from bacterial extracts under mild conditions.
Protein tagging has become a widely applied method for ready purification of proteins.
The specific interaction between the tag and a solid-phase ligand allows for the selective binding and elution of the tagged protein.
Many vectors that incorporate these tags have been engineered with a cleavage site between the tag and the protein of interest.
This permits the separation of the protein from the tag, if needed.
One commonly used system is the maltose-binding protein (MBP).
In this system, the target gene is inserted in the appropriate vector and expressed.
Following purification using an amylose column, the MBP-target protein complex can be eluted from the column with maltose and cleaved with a specific protease to yield the purified target protein.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000Western Blot: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes Maltose Binding Protein Epitope tag.
