Cyclic adenosine monophosphate or cAMP is an intracellular by-product of ATP by adenylene cyclase when G-protein Coupled Receptors (GPCR) bind various ligands on the cell surface.
Cyclic AMP acts as a second messenger for signal transduction across the cell membrane and is a part of kinase activation.
It regulates the effects of glucagon and adrenaline along with the movement of calcium ions through ion channels.
Since it is affected directly by GPCR activation, cAMP is studied as a means to monitor GPCR in the discovery of therapeutic drugs.
Applications:Suitable for use in RIA and ELISA.
Other applications not tested.
Recommended Dilution:RIA: 1:6400Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
