HRD1, a Ubiquitin-protein ligase (E3), is required for the degradation and ubiquitination of several endoplasmic reticulum-associated degradation (ERAD) substrates and physically associates with relevant ubiquitin-conjugating enzymes (E2s).
Human HRD1 is localized to the ER and ubiquitinates its substrates.
HRD1 has been suggested to be up-regulated to protect against ER stress-induced apoptosis by degrading unfolded proteins accumulated in the ER.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 5-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human SYVN1/HRD1. Species Crossreactivity: bat, gibbon, bovine, canine, xenopus, gorilla, hamster, equine, monkey, mouse, rabbit, rat and zebrafish.
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Bo Ca Eq Hm Hu Mk Mo Rb Rt Xe Ze
Immunogen：Synthetic peptide corresponding to 18aa from the internal region of human SYVN1/HRD1.
