DAX-1 (DSS-AHC critical region on the X chromosome, gene 1) is an orphan NRO Knirps-Like nuclear receptor that is involved in adrenal gland and gonad development.
DAX-1 has been shown to negatively regulate transcription of the nuclear receptor steroidogenic factor 1 (SF-1).
DAX-1 is also involved in the development and differentiation of anterior pituitary cells and in steroidogenesis.
In the nucleus, DAX-1 binds to a retinoic acid-responsive element (RARE) and acts as a dominant-negative regulator of retinoic acid receptor-mediated transcription.
DAX-1 gene transcription is regulated by Ad4BP/SF-1 (NR5A1) and is suppressed by androgens and the androgen receptor (AR/NR3C4); AR down-regulated DAX-1 gene transcription is mediated by Ad4BP/SF-1 in the presence of the ligand.
Recently it has been shown that DAX-1 is required for testis development (Meeks et al.
2003).
Mutations in the human DAX-1 gene lead to X-linked adrenal hypoplasia congenita and hypogonadotropic hypogonadism.
Recently two isoforms of DAX-1 has been identified.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 11ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human NR0B1/DAX1. Species Crossreactivity: monkey, chimpanzee, orangutan
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu Mk
Immunogen：Synthetic peptide corresponding to 20aa from the C-terminus of human NR0B1/DAX1. Species sequence homology: gorilla and gibbon.
