NEK9, a NEK type protein kinase, regulates chromosome alignment and segregation in mitosis.
The protein has a N-terminal NIMA-like catalytic domain, a central domain with homology to the guanine nucleotide exchange factor for the GTPase Ran (RCC1), and a C-terminal coiled-coil domain.
It is phosphorylated by active p34(Cdc2) and is capable of autophosphorylation and oligomerization.
The activated protein binds Nek6 and RCC1, and has been shown to associate with the human homolog of the Drosophila protein Bicaudal D (Bicd2) in vivo and to phosphorylate this coiled-coil protein in vitro.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 4ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human NEK9. Species Crossreactivity: monkey, mouse, bovine, equine, porcine, rabbit, chicken, gorilla, gibbon, bat
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Bo Ch Eq Hu Mk Mo Po Rb
Immunogen：Synthetic peptide corresponding to 20aa from human NEK9. Species sequence homology: canine, xenopus and rat.
