NEK6 encodes a NEK type protein kinase, which contains a C-terminal NIMA-like catalytic domain.
The protein is believed to regulate chromatin condensation in mammalian cells via phosphorylation of histones H1 and H3, and it has been shown to phosphorylate ribosomal S6 protein kinase (S6K1) and serum and glucocorticoid induced protein kinase (SGK1), which regulate diverse cellular processes.
The protein also has been shown to bind NEK9, another NIMA-related kinase which regulates chromosome alignment and segregation.
At least three mRNA transcripts have been identified, 1.6-, 2.6-, and 9.5-kb.
The NEK6 gene is localized to 9q33-34, a region at which the loss of heterozygosity is associated with transitional cell carcinomas.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human NEK6. Species Crossreactivity: monkey, gorilla, orangutan and gibbon.
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu Mk
Immunogen：Synthetic peptide corresponding to 14aa from the N-terminus of human NEK6.
