The prion protein is a large membrane protein that occurs normally in neurons of the human brain.
It is thought to be involved in synaptic transmission.
In prion diseases (such as CJD, Gerstmann-Straussler-Scheinker syndrome (GSS), Fatal Familial Insomnia (FFI), Alpers Syndrome and Kuru) the normal cellular form of this protein (PrPc) is transformed into an altered protein when it comes into contact with an infectious prion protein (PrPsc) from another host.
This altered PrPsc accumulates in cytoplasmic vesicles of diseased individuals forming lesions, vacuoles and amyloid deposits.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:20000-1:50000Western Blot: 1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 20mM Tris-HCl, 10mg/ml BSA, pH 8.0, 0.05% sodium azide.
Specificity：Recognizes human prion. Species crossreactivity: sheep, bovine, mouse.
Clone # USB：C16-S
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Hu Mo Sh
Immunogen：Peptide corresponding to the C-Terminus of third a-helical domain of human prion protein.
