84-2138-16　Topoisomerase I (Alpha-DNA Topoisomerase I, DNA Topoisomerase I) 50ug　169586

特徴

• Human DNA Topoisomerase I (Topo I) is a monomeric protein of 765 anino acids encoded by a single-copy gene.
• Based on its function, DNA Topoisomerase I has been subdivided into four distinct domains.
• The N terminal 214 amino acids of Topo I comprise a highly charged N-terminal domain involved in protein-protein interactions with a number of cellular proteins.
• The highly conserved core domain expanded from amino acids 215 to 636 contains most catalytic residues and retains DNA binding activity.
• The active C-terminal domain (residues 713-765) is connected to the core domain by a poorly conserved linker domain (residues 636-712).
• Human DNA Topoi- somerase I is the best studied of the DNA Topoisomerase family.
• It catalyzes the relaxation of both positive and negative supercoiled DNAs without the requirement of energy.
• In addition to DNA replication and transcriptional activa- tion, DNA Topoisomerase I also plays a major role in pre-mRNA splicing, cell cycle and other gene regulatory pathways during cell growth and development.
• Since Topo I can cause large amounts of single-strand DNA breaks and this kind of DNA damage closely relates to most cancer, it is therefore believed that DNA Topo I can be an important target for antitumor agents.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:200-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.