Transcription factor IIF (TFIIF) is a heterodimer, com- posed of 58kD (RAP74) and 26kD (RAP30) subunits.
It was first identified through the ability to interact with immobilized RNA polymerase II.
In addition to its role in transcription initiation, TFIIF can increase the specificity and efficiency of RNA polymerase II transcription, and can especially increase the rate of transcription elongation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:200-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS.
Specificity：Recognizes human TFIIF. Reacts with the RAP74 protein in HeLa nuclear extract.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Rb
Immunogen：Recombinant protein full-length RAP74 subunit of the TFIIF complex.
