Transcription factor IIF (TFIIF) is composed of 58kD (RAP74) and 26kD (RAP30) subunits as a heterodimer, and was first identified through the ability to interact with immobilized RNA polymerase II.
The RAP30 subunit of TFIIF contains two distinct regions with sequence similarity to E.
coli factors and can deliver RNA polymerase II to the promoter to support transcription initiation in the absence of RAP74.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:200-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS.
Specificity：Recognizes human TFIIF. Reacts with the RAP30 protein in HeLa nuclear extract.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Rb
Immunogen：Recombinant protein corresponding to full-length RAP30 subunit of the TFIIF complex.
