Sp1 was the first transcription factor to be cloned and characterized.
Analysis of structure and function has re- vealed that Sp1 can be separated into discrete functional domains.
The DNA-binding domain consists of three zinc fingers that specifically bind to the GC-box element.
Sp1 contains at least four separate transcriptional activation domains.
Two of these domains are glutamine-rich, a well- characterized motif found in several other transcription factors.
In addition to transcription, Sp1 has been linked to cell growth, cancer, Huntington disease and other disor- ders through transcriptional regulation or specific protein-protein interactions.
The function of Sp1 can be regulated by phosphorylation and glycosylation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum.
Form：Supplied as a liquid in PBS.
Specificity：Recognizes human Sp1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Rb
Immunogen：Recombinant protein corresponding to Sp1 generated from baculovirus system.
