hRPB5 (p33) is a highly conserved subunit shared by all three RNA polymerases.
It has been shown to be in close contact to promoter DNA when Pol II is recruited into the preinitiation complex.
RPB5 has also been implicated in direct protein-protein contacts with transcription factor IIB, RAP30 subunit of transcription factor IIF, and gene-specific modulator proteins, such as the hepatitis B virus transactivator protein X or an inhibitor of Pol II, RMP (RPB5-mediating protein).
Therefore, RPB5 is facilitating the communication between the Pol II core and a variety of basal and gene-specific transcription factors.
In the Pol II complex, RPB5 interacts with RPB3 and the RPB3–RPB5 interaction is intensified in the presence of three subunits, RPB7, RPB8 and RPB11.
RPB5 also makes direct contacts with both RPB1 and RPB2, the two large subunits of the polymerase complex.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:200-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS
Specificity：Recognizes RNA Pol II. Reacts with the p33 protein in HeLa nuclear extract.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Rb
Immunogen：Recombinant protein corresponding to full-length p33 subunit of the polymerase complex.
