Retinoid X receptor (RXR) serves as a promiscuous heterodimerization partner for many nuclear receptors through the identity box, a 40-amino acid subregion within the ligand binding domain (LBD).
RXR partners include thyroid hormone receptors (TRs), retinoic acid receptors (RARs), peroxisome proliferator-activated re-ceptor, several constitutive active orphan nuclear receptors (e.g.
RXRs also form homodimers to mediate the effects of 9-cis-retinoic acid (9-cRA).
Depending on these protein-protein interactions, RXR- containing complexes have distinct ligand-dependent and constitutive functions.
The LBD is functionally complex and mediates ligand binding, receptor homo- and heterodimerization, repression of transcrip-tion in the absence of lig- and, and ligand-dependent ac-tivation of transcription.
Hormone binding to the struc-turally conserved LBD of the RXR triggers a conformational change that principally affects the conserved C-terminal transactivation helix H12 involved in transcriptional activation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:200-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS.
Specificity：Recognizes a-RXR. Reacts with the RXR protein in HeLa nuclear extract.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Rb
Immunogen：Recombinant protein corresponding to ligand-binding domain of RXR.
