Methionine sulfoxide reductases (Msrs) are repair enzymes that protect proteins from oxidative stress by catalyzing stereospecific reduction of oxidized methionine residues.
MsrB1 is a selenocysteine-containing cytosolic/nuclear Msr with high expression in liver and kidney.
MsrB1 occurs in two protein forms that migrate as 14 and 5kD proteins.
Each mRNA splice form generated both protein forms (Liang X et.al.
2010).
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum.
Form：Supplied as a liquid in 50% glycerol.
Specificity：Recognizes human MsrB1. Antibody specificity was evaluated by indirect immunofluorescence and western blotting. Western blot of 50ug protein of the human oral mucosa, parathyroid and thyroid gland revealed in each case the protein band of the 13kD MsrB1. Anti-MSRB1 is without crossreactivity to the other MSRs (MSRA, MSRB2 and MSRB3). The antibody visualized the MSRB1- immunoreactivity into the cytoplasma of thyroid gland cells (unpublished Fig 1a). The nuclei are stained with DAPI (Fig.:1 b). In Fig. 1c is merged 1a and 1b).
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human MsrB1.
