Mitogen-activated protein (MAP) kinase pathway has been implicated in the actions of many cellular processes including hormones, growth factors and oncogene products.
The mitogen-activated protein kinase kinase 1 (MEK1 or MAPKK1) is one of the three isoforms of the protein serine/threonine kinase family.
The mechanism of MEK1 activation involves protein phosphorylation specifically on serine 218 and 222 by MEK kinase (MEKK) or by autophosphorylation.
Activation of MEK1 in vivo was also demonstrated through the inhibition of serine/threonine phosphayases by SV40 smal T antigen, an inhibitor of phosphatase PP2A.
Oncogene products including Raf, Tpl-2 and Ras have been demonstrated to be involved in mediating MEK1 activity.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：125ul
Host：rabbit
Grade：Serum
Purity：Serum.
Form：Supplied as a liquid.
Specificity：Recognizes MEK1.
Isotype：IgG
Calc Applications Abbrev：WB
Immunogen：Recombinant protein corresponding to full-length MEK1.
