84-2137-90　MDM2 (Murine Double Minute-2) 50ug　169556

特徴

• Originally discovered as one of three genes amplified on double minute chromosomes in a tumorigenic derivative of NIH 3T3 cells, MDM2 was later shown to possess oncogenic potential when overexpressed.
• High-level expression of MDM2 has also been shown to confer tumori- genic potential upon nontransformed rodent fibroblasts in athymic nude mice.
• MDM2 can immortalize rat embryo fibroblasts and can cooperate with activated RAS to transform these cells.
• Elevated levels of MDM2 protein have been found in a variety of human tumors, most notably in soft tissue sarcomas where up to 30% of primary tumors contain multiple copies of the MDM2 gene.
• One mechanism by which MDM2 overexpression promotes tumor development is through its ability to bind to the p53 tumor suppressor, thereby blocking the transactivation, cell cycle arrest (8), and apoptotic functions of p53 (9).
• MDM2 can inhibit p53 activity in a number of ways including preventing p53 from recruiting TAFs, promoting nuclear export, inhibiting p53 acetylation, and perhaps most im- portantly by virtue of its function as an E3 ubiquitin ligase with specificity for, among others, p53 (reviewed in 10).
• In addition to regulating p53 levels by targeting p53 for proteasomal degradation MDM2 also transfers ubiquitin to itself, MDMX, the s2 adrenergic receptor, glucocorti- coid receptor, TIP60, and PCAF (10).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:2000-1:5000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.