Glucokinase, also termed Hexokinase D, is the characteristic isoenzyme of hexokinase in pancreatic islet ̈s-cells (IGLK, B-type GK; GKB) and in the liver (L-Type GK; GKL) that phosphorylates glucose at the sixth carbon position, thus committing glucose to the glycolytic pathway.
The protein localizes to the outer membrane of mitochondria.
In contrast with other forms of hexokinase, GK is not inhibited by its product (glucose-6-phosphate) but remains active while glucose is abundant.
In B-type GK-containing cells, glucose is the predominant inducer of GK.
Glucose-driven GK expression is modified by many positive and negative factors: it is augmented by cAMP, biotin, retinoic acid, PL, and insulin, and it is decreased by low cytosolic Ca2+ levels.
Even though insulin is not sufficient to induce the enzyme, the hormone may still be required for optimal GK expression in B-type GK-containing cells.
To date, over 150 GK gene mutations have been found that are manifest in at least three clearly distinguishable syndromes inherited in an autosomal dominant manner: 1) GK-linked persistent hyperinsulinemic hypoglycemia (PHHI-GK); 2) GK-linked permanent neonatal diabetes (PNDM-GK); and 3) GK-linked maturity-onset diabetes of the young (MODY-GK, also called MODY-2).
Recently, a novel class of drugs that stimulate the GK molecule directly have been discovered, which offer a new principle for drug therapy of diabetes.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Grade：Purified
Purity：Purified.
Form：Supplied as a liquid in PBS.
Specificity：Recognizes IGLK.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Rb
Immunogen：Recombinant protein corresponding to full-length IGLK.
