FMRFamides belong to the most extensively studied neuropeptides in invertebrates and exhibit diverse physiological effects on different target organs, such as muscles, intestine and the nervous system.
This study on the American cockroach confirms for the first time that extended FMRFamides occur in non-dipteran insects.
By means of tandem mass spectrometry, these neuropeptides were structurally elucidated, and sequence information was used for subsequent cloning of the cockroach FMRFamide gene.
This precursor gene encodes for 24 putative peptides and shows sufficient similarity with the Drosophila FMRFamide gene.
Of the 24 peptides, 23 were detected by mass spectrometric methods; it is the highest number of neuropeptide forms shown to be expressed from a single precursor in any insect.
The expression was traced back to single neurons in the thoracic ganglia.
The unique accumulation of these FMRFamide-related peptides in thoracic perisympathetic organs provides the definite evidence for a tagma-specific distribution of peptidergic neurohormones in neurohaemal release sites of the insect CNS.
Excitatory effects of the cockroach FMRFamides were observed on antenna–heart preparations.
In addition, the newly described FMRFamides reduce the spike frequency of dorsal-unpaired median neurons and reduce the intracellular calcium concentration, which may affect the peripheral release of the biogenic amine octopamine.
Applications:Suitable for use in ELISA and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：20ul
Host：rabbit
Grade：Serum
Purity：Serum.
Form：Supplied as a liquid in 50% glycerol.
Specificity：Recognizes head-peptide. In insects the FMRFamide-related peptides (FaRPs) are grouped into different families, which are expressed from different precursor genes: the FMRFamides, the myosuppressins, the sulfakinins and the head peptides. Cross-reactivity to the other FaRPs is visible, but the pattern of the head peptide immunoreactivity from brain, ventral nerve cord, midgut and antenna-heart (Fig 1,2) is different to tested RF-amide sera (FMRFamide, anti- Perisulfakinin).
Isotype：IgG
Calc Applications Abbrev：E IC
Immunogen：Periplaneta americana midgut neuropeptide, ANRSPSLRLRLRFamide, coupled to bovine thyroglobulin using glutaraldehyde.
