Dr1 is a general negative regulator of class II and class III gene expression.
It binds to the basic repeat domain of TBP on promoter DNA and can prevent the RNA polymerase II holoenzyme, or its TFIIB and/or TFIIA subunits, from assembling into an initiation complex.
Dr1 is phosphorylated in vivo and this modification affects its interaction with TBP.
In addition, Dr1 interacts with the hyperphosphorylated form of Pol II and with the repression domain of the AREB6 repressor.
Dr1 forms a heterodimer complex with DRAP1 through its histone fold domain.
More recently it was discovered that Dr1-DRAP1 is a bi-functional basal transcription factor that differentially regulates gene transcription through DPE (downstream pro- moter elements) or TATA box motifs.
It can stimulate transcription in vitro from Drosophila promoters contain- ing DPEs, whereas it represses transcription from TATA- containing promoters.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS.
Specificity：Recognizes Dr1. Reacts with the Dr1 protein in HeLa nuclear extract.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Rb
Immunogen：Recombinant protein corresponding to full-length Dr1 protein.
