The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
Applications:Suitable for use in RIA, Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:50-1:500Western Blot: 1:50-1:500Immunohistochemistry: 1:50-1:500 Immunocytochemistry: 1:50-1:500 Dot Blot: 1:50-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：sheep
Source Antibody：rabbit
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder in PBS, pH 7.2. No preservative added. Reconstitute with 1ml sterile dH2O. Labeled with Biotin.
