The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques); to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of the customer’s own choice.
Applications:Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot and Dot Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:10-1:300ELISA: 1:10-1:300Western Blot: 1:10-1:300Dot Blot: 1:10-1:300Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：5mg
Host：sheep
Source Antibody：rabbit
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2. Reconstitute with 1ml sterile dH2O PBS.
