Factor XII (Hageman factor) is a single chain glycoprotein (MW 80,000).
It is a serine protease zymogen.
FXII is converted to an active enzyme by damage to vascular endothelium.
In vitro activation is caused by glass or kaolin.
Surface binding promotes activation by kallikrein.
FXIIa activates prekallikrein and FXI.
Kallikrein activates more FXII.
Fragments of FXIIa activate FVII to FVIIa, and plasminogen to plasmin.
The complement system is also activated by FXIIa.
A link between fibrin clot and the subendothelium is formed by FXIIa crosslinking with fibrin, alpha-2 plasmin inhibitor, fibronectin and Van Willebrand factor.
Applications:Suitable for use in Immunoelectrophoresis, Bi-dimensional Electrophoresis, Radial Immunodiffusion and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：goat
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 0.09% sodium azide. Reconstitute with 1ml sterile dH2O.
Specificity：Recognizes human Factor XII. In immunoelectrophoresis a reaction with Rhesus Monkey plasma has been found. Does not react with other varients of human plasma proteins.
